1ZW3

Vinculin Head (0-258) in Complex with the Talin Rod residues 1630-1652

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-28
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 21 21 2
Unit cell lengths	51.621, 72.168, 96.725
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.300
R-factor	0.22808
Rwork	0.224
R-free	0.30723
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xwj
RMSD bond length	0.026
RMSD bond angle	2.286
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.078	0.378
<I/σ(I)>	10.85	3.03
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.1	293	1.2M NaH2PO4/0.8M K2HPO4, 100mM CAPS, pH 10.5, pH 6.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K