1ZVY
Crystal structure of the VHH D3-L11 in complex with hen egg white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.84530 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.913, 49.879, 56.995 |
| Unit cell angles | 90.00, 92.08, 90.00 |
Refinement procedure
| Resolution | 17.740 - 1.630 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jtt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.087 | 0.354 |
| Number of reflections | 29801 | |
| Completeness [%] | 99.5 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | magnesium acetate, sodium cacodylate, PEG 8000, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
