1ZVV
Crystal structure of a ccpa-crh-dna complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.998 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.690, 158.100, 125.470 |
| Unit cell angles | 90.00, 100.73, 90.00 |
Refinement procedure
| Resolution | 79.050 - 2.980 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rzr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.100 | 3.120 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.492 | |
| Number of reflections | 32545 | |
| <I/σ(I)> | 6 | 2 |
| Completeness [%] | 92.0 | 92 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 3350, sodium iodide, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 2 | PEG 3350 | ||
| 3 | 1 | 2 | NaI |
