1ZVU
Structure of the full-length E. coli ParC subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1157 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 257.990, 62.141, 63.998 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.24323 |
| Rwork | 0.240 |
| R-free | 0.29583 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1AB4 (E. coli GyrA NTD) related entry 1ZVT (E. coli ParC CTD) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.087 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 19320 | |
| <I/σ(I)> | 9.5 | 2.5 |
| Completeness [%] | 89.7 | 89.1 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES, NaCl, glycerol, PEG-6000, 1,3-butanediol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
