1ZVP
Crystal Structure of a Protein of Unknown Function VC0802 from Vibrio cholerae, Possible Transport Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-21 |
Detector | SBC-2 |
Wavelength(s) | 0.9798 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 97.224, 99.561, 163.433 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 81.650 - 2.200 |
R-factor | 0.21573 |
Rwork | 0.214 |
R-free | 0.24643 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.327 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.077 | 0.720 |
Number of reflections | 40583 | |
<I/σ(I)> | 17.44 | 1.28 |
Completeness [%] | 95.1 | 72.2 |
Redundancy | 4.5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 45% glycerol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |