1ZVB
A structure-based mechanism of SARS virus membrane fusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-13 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 58.908, 34.405, 51.718 |
Unit cell angles | 90.00, 93.91, 90.00 |
Refinement procedure
Resolution | 51.600 - 1.700 |
R-factor | 0.196 |
Rwork | 0.194 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zv8 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.312 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.600 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.036 | 0.210 |
Number of reflections | 11231 | |
<I/σ(I)> | 21.1 | 5.1 |
Completeness [%] | 96.9 | 94.7 |
Redundancy | 2.9 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | PEG 4000, isopropanol, sodium citrate, pH 5.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K |