1ZV9
Crystal structure analysis of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus- SeMet derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 53.858, 53.858, 111.964 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.630 - 1.280 |
R-factor | 0.118 |
Rwork | 0.116 |
R-free | 0.15800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qzn |
RMSD bond length | 0.015 |
RMSD bond angle | 1.472 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.300 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmerge | 0.065 | 0.490 |
Number of reflections | 48796 | |
<I/σ(I)> | 24.2 | 2.6 |
Completeness [%] | 98.9 | 97.1 |
Redundancy | 12.8 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 293 | Ammonium sulfate, pH 6, vapor diffusion, temperature 293K |