1ZV4
Structure of the Regulator of G-Protein Signaling 17 (RGSZ2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 105.153, 105.153, 57.515 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.700 - 2.400 |
| R-factor | 0.22935 |
| Rwork | 0.228 |
| R-free | 0.26204 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cmz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.187 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.560 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.072 | 0.421 |
| Number of reflections | 7589 | |
| <I/σ(I)> | 23 | 4.2 |
| Completeness [%] | 98.4 | 100 |
| Redundancy | 9.9 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | mPEG2K, Na succinate, Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
