1ZV2
Cu-containing nitrite reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-28 |
Detector | MARRESEARCH |
Wavelength(s) | 1.007 |
Spacegroup name | H 3 |
Unit cell lengths | 72.381, 72.381, 147.509 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.700 - 1.740 |
R-factor | 0.15667 |
Rwork | 0.155 |
R-free | 0.19367 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nif |
RMSD bond length | 0.012 |
RMSD bond angle | 1.438 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.700 | 1.810 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.065 | 0.340 |
Number of reflections | 29034 | |
<I/σ(I)> | 9.7 | 2.9 |
Completeness [%] | 98.6 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 295 | PEG4000, MgCl2, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |