1ZUZ
Calmodulin in complex with a mutant peptide from human DRP-1 kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.813 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 66.520, 34.030, 73.850 |
Unit cell angles | 90.00, 109.58, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.910 |
R-factor | 0.1941 |
Rwork | 0.191 |
R-free | 0.24829 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wrz |
RMSD bond length | 0.010 |
RMSD bond angle | 1.115 |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.000 |
High resolution limit [Å] | 1.910 | 1.910 |
Number of reflections | 12230 | |
<I/σ(I)> | 19.9 | 3.8 |
Completeness [%] | 99.0 | 97.5 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 295 | PEG3350, MPD, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |