1ZUN
Crystal Structure of a GTP-Regulated ATP Sulfurylase Heterodimer from Pseudomonas syringae
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2004-08-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9184, 0.9793 |
Spacegroup name | H 3 |
Unit cell lengths | 110.072, 110.072, 171.002 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.22439 |
Rwork | 0.222 |
R-free | 0.27617 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.059 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.066 | 0.346 |
Number of reflections | 21160 | |
<I/σ(I)> | 16.07 | 2.84 |
Completeness [%] | 0.2 | 98.5 |
Redundancy | 5.8 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | PEG 4000, tris hydrochloric acid, sodium acetate, guanosine diphosphate, 5'-adenyly-diphosphate-monothiophosphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | PEG 4000, tris hydrochloric acid, sodium acetate, guanosine diphosphate, 5'-adenyly-diphosphate-monothiophosphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |