1ZUK
Yeast BBC1 Sh3 domain complexed with a peptide from Las17
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.813 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 51.770, 78.500, 35.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.17645 |
| Rwork | 0.174 |
| R-free | 0.22599 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tg0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.279 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.950 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 12659 | |
| <I/σ(I)> | 15.3 | 2.8 |
| Completeness [%] | 98.4 | 98.5 |
| Redundancy | 4.3 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | VAPOR DIFFUSION, HANGING DROP, temperature 295K |
