1ZUJ

The crystal structure of the Lactococcus lactis MG1363 DpsA protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	298
Detector technology	CCD
Collection date	2001-12-05
Detector	ADSC QUANTUM 4
Wavelength(s)	1.488
Spacegroup name	H 3 2
Unit cell lengths	131.840, 131.840, 325.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.900
R-factor	0.25622
Rwork	0.253
R-free	0.32216
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zs3
RMSD bond length	0.014
RMSD bond angle	1.566
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	TNT
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.078	0.382
Number of reflections	22949
<I/σ(I)>	6.7	1.7
Completeness [%]	94.5	93.8
Redundancy	2.6	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1.7M ammonium sulphate, 0.1M HEPES/Na, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1.7M ammonium sulphate, 0.1M HEPES/Na, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1.7M ammonium sulphate, 0.1M HEPES/Na, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K