1ZU5

Crystal structure of FtsY from Mycoplasma mycoides- space group H32

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-03-03
Detector	MARRESEARCH
Wavelength(s)	0.91
Spacegroup name	H 3 2
Unit cell lengths	148.441, 148.441, 223.925
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.21156
Rwork	0.210
R-free	0.24642
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB CODE 1ZU4
RMSD bond length	0.018
RMSD bond angle	1.674
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	35370
Completeness [%]	95.0	98.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	Sodium citrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K