1ZTW
d(CTTAATTCGAATTAAG) complexed with Moloney Murine Leukemia Virus Reverse Transcriptase catalytic fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2004-04-15 |
Detector | SBC-3 |
Wavelength(s) | 0.97951 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 54.934, 145.746, 46.855 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.700 - 1.800 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.193 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 35445 | |
Completeness [%] | 98.9 | 97.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 4000, magnesium acetate, ADA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | magnesium acetate | ||
3 | 1 | 1 | ADA | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | PEG 4000 | ||
6 | 1 | 2 | magnesium acetate | ||
7 | 1 | 2 | ADA | ||
8 | 1 | 2 | H2O |