1ZTV

Crystal structure of a duf72 family protein (ef0366) from enterococcus faecalis v583 at 3.10 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-06
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	91.819, 92.358, 119.347
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.130 - 3.100
R-factor	0.19257
Rwork	0.191
R-free	0.22400
Structure solution method	MR
Starting model (for MR)	1vpy
RMSD bond length	0.011
RMSD bond angle	0.647
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	119.520	119.520	3.270
High resolution limit [Å]	3.100	9.800	3.100
Rmerge	0.080	0.019	0.392
Number of reflections	18876
<I/σ(I)>	8.6	25.1	1.9
Completeness [%]	99.4
Redundancy	3.4	3.1	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	6.5	277	1.0M NaCitrate, 0.1M Cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K