1ZTU
Structure of the chromophore binding domain of bacterial phytochrome
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 98 |
Detector technology | CCD |
Collection date | 2004-10-25 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9793, 0.9791 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 64.876, 133.666, 49.938 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.23887 |
Rwork | 0.237 |
R-free | 0.26569 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.817 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.056 | 0.111 |
Number of reflections | 15209 | |
<I/σ(I)> | 14.2 | 10.8 |
Completeness [%] | 97.0 | 90.4 |
Redundancy | 6.7 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.95 | 298 | PEG 400, sodium acetate, pH 4.95, VAPOR DIFFUSION, temperature 298K |
1 | VAPOR DIFFUSION | 4.95 | 298 | PEG 400, sodium acetate, pH 4.95, VAPOR DIFFUSION, temperature 298K |