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1ZTU

Structure of the chromophore binding domain of bacterial phytochrome

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 32-ID
Synchrotron siteAPS
Beamline32-ID
Temperature [K]98
Detector technologyCCD
Collection date2004-10-25
DetectorMARRESEARCH
Wavelength(s)0.9793, 0.9791
Spacegroup nameP 21 21 2
Unit cell lengths64.876, 133.666, 49.938
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 2.500
R-factor0.23887
Rwork0.237
R-free0.26569
Structure solution methodMAD
RMSD bond length0.010
RMSD bond angle1.817
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareSHARP
Refinement softwareREFMAC
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.590
High resolution limit [Å]2.5002.500
Rmerge0.0560.111
Number of reflections15209
<I/σ(I)>14.210.8
Completeness [%]97.090.4
Redundancy6.75.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION4.95298PEG 400, sodium acetate, pH 4.95, VAPOR DIFFUSION, temperature 298K
1VAPOR DIFFUSION4.95298PEG 400, sodium acetate, pH 4.95, VAPOR DIFFUSION, temperature 298K

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