1ZTP

X-ray structure of gene product from homo sapiens Hs.433573

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-05
Detector	ADSC
Spacegroup name	P 21 21 21
Unit cell lengths	62.547, 116.809, 123.635
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.19118
Rwork	0.188
R-free	0.24500
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.422
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.106	0.597
Number of reflections	31664
<I/σ(I)>	13.353	2.675
Completeness [%]	98.6	93.1
Redundancy	12.3	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	10 MG/ML PROTEIN, 1.2 M SODIUM CITRATE, 0.100 M TRIS, pH 8.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K