1ZT7
crystal structure of class I MHC H-2Kk in complex with a nonapeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93300 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 85.135, 72.628, 88.793 |
| Unit cell angles | 90.00, 111.24, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.23474 |
| Rwork | 0.231 |
| R-free | 0.31074 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | the complex between H-2Kk and the octapeptide HA(259-266) |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.210 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 18302 | |
| Completeness [%] | 90.2 | 90.4 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.6M ammonium sulfate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
