1ZSP
Contribution to Structure and Catalysis of Tyrosine 34 in Human Manganese Superoxide Dismutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-15 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 79.515, 79.515, 242.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.560 - 1.900 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1N0J.PDB |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.560 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.152 |
| Number of reflections | 31760 | |
| <I/σ(I)> | 48.9 | 11.5 |
| Completeness [%] | 91.2 | 94.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 2.5 M AMMONIUM SULFATE, 100MM IMIDAZOLE, pH 7.50, VAPOR DIFFUSION, temperature 293K |
