1ZSH

Crystal structure of bovine arrestin-2 in complex with inositol hexakisphosphate (IP6)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-02
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 32 2 1
Unit cell lengths	78.880, 78.880, 158.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.800 - 2.900
Rwork	0.250
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jsy
RMSD bond length	0.013
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.800	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	14737
<I/σ(I)>	17.8
Completeness [%]	99.6	97.7
Redundancy	16.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	Magnesium Formate, HEPES, Polypropylene glycol 400, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K