1ZSC

CARBONIC ANHYDRASE II MUTANT E117Q, HOLO FORM

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-03-14
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	42.700, 41.700, 73.000
Unit cell angles	90.00, 104.60, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.177
Rwork	0.177
RMSD bond length	0.015
RMSD bond angle	26.000 *
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	1.800
Rmerge	0.070
Total number of observations	49030 *
Number of reflections	22924
Completeness [%]	92.0
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *	25 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	50 (mM)
2	1	reservoir		50-75 (%sat)
3	1	drop	protain	0.3 (mM)
4	1	drop	Tris-HCl	50 (mM)