Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-09 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9000, 0.9798, 0.9800 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.017, 57.546, 87.318 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.700 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.24100 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.520 |
Data reduction software | AUTOMAR |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.770 |
High resolution limit [Å] | 1.700 | 1.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | PEG4000, Hepes, pH 8.50, VAPOR DIFFUSION, HANGING DROP, temperature 291K |