1ZS3

The crystal structure of the Lactococcus lactis MG1363 DpsB protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	298
Detector technology	CCD
Collection date	2001-05-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	127.977, 128.379, 193.903
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.20941
Rwork	0.207
R-free	0.25779
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qgh
RMSD bond length	0.022
RMSD bond angle	1.692
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CCP4
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.074	0.407
Number of reflections	85912
<I/σ(I)>	7.5	1.7
Completeness [%]	97.8	97.4
Redundancy	3.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	2.0M Sodium Formate, 0.1M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	2.0M Sodium Formate, 0.1M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K