1ZS2
Amylosucrase Mutant E328Q in a ternary complex with sucrose and maltoheptaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2000-05-02 |
Detector | MARRESEARCH |
Wavelength(s) | 0.983 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 95.741, 116.358, 61.051 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.220 - 2.160 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g5a |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.240 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.095 | 0.238 |
Number of reflections | 37336 | |
<I/σ(I)> | 13 | 4.3 |
Completeness [%] | 99.2 | 96.7 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 6000, SODIUM CHLORIDE, TRIS-HCL, EDTA, HEPES, DTT, sucrose, maltoheptaose, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |