1ZRH
Crystal structure of Human heparan sulfate glucosamine 3-O-sulfotransferase 1 in complex with PAP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-05-16 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 58.887, 135.255, 48.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.570 - 2.100 |
R-factor | 0.1849 |
Rwork | 0.184 |
R-free | 0.20911 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vkj |
RMSD bond length | 0.019 |
RMSD bond angle | 1.689 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.570 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.114 | 0.551 |
Number of reflections | 22876 | |
<I/σ(I)> | 8.5 | 3.04 |
Completeness [%] | 98.5 | 98.1 |
Redundancy | 5.1 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 300 | 2.0 - 2.4 M Sodium Formate, 0.1 M Sodium-Acetate pH 4.6, 5% Glycerol, VAPOR DIFFUSION, temperature 300K |