1ZRH
Crystal structure of Human heparan sulfate glucosamine 3-O-sulfotransferase 1 in complex with PAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.887, 135.255, 48.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.570 - 2.100 |
| R-factor | 0.1849 |
| Rwork | 0.184 |
| R-free | 0.20911 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vkj |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.689 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.570 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.114 | 0.551 |
| Number of reflections | 22876 | |
| <I/σ(I)> | 8.5 | 3.04 |
| Completeness [%] | 98.5 | 98.1 |
| Redundancy | 5.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 300 | 2.0 - 2.4 M Sodium Formate, 0.1 M Sodium-Acetate pH 4.6, 5% Glycerol, VAPOR DIFFUSION, temperature 300K |
