1ZR4
Structure of a Synaptic gamma-delta Resolvase Tetramer Covalently linked to two Cleaved DNAs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.990 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 119.158, 127.290, 140.419 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.400 |
R-factor | 0.26554 |
Rwork | 0.264 |
R-free | 0.29469 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.453 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.550 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.047 | 0.010 |
Number of reflections | 33091 | |
<I/σ(I)> | 25.7 | 2 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 8 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 303 | 0.2M NaCl, 0.1M Magnesium Formate, 0.1M Sodium Acetate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Sodium Chloride | ||
2 | 1 | 1 | Magnesium Formate | ||
3 | 1 | 1 | Sodium Acetate | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | Sodium Chloride | ||
6 | 1 | 2 | Magnesium Formate | ||
7 | 1 | 2 | Sodium Acetate | ||
8 | 1 | 2 | H2O |