1ZQZ
DNA POLYMERASE BETA (POL B) (E.C.2.7.7.7), 31-KD DOMAIN; SOAKED IN THE PRESENCE OF MNCL2 (50 MILLIMOLAR)
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1996-01-10 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 118.565, 62.728, 37.458 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.202 * |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | 1zqu |
RMSD bond length | 0.021 |
RMSD bond angle | 22.200 * |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | TNT (V. 5-D) |
Refinement software | TNT (5-D) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.079 * | 0.211 * |
Number of reflections | 7763 | |
<I/σ(I)> | 8.9 | 2.1 |
Completeness [%] | 95.0 | 84 |
Redundancy | 2.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 | THIS ENTRY DESCRIBES THE STRUCTURE THAT RESULTED WHEN A CRYSTAL OF THE C-TERMINAL 31-KD DOMAIN OF RAT DNA POL BETA (SEE ENTRY 1BPB AND REFERENCE 9) HAD BEEN SOAKED IN THE FOLLOWING SOLUTION FOR 24 HOURS: PEG 3350, 16% TRIS-HCL, 100 MILLIMOLAR, PH 7.5 MNCL2, 50 MILLIMOLAR SEE REFERENCE 3 FOR DETAILS CONCERNING EXPERIMENTAL PROCEDURES, RESULTS, AND DISCUSSION FOR THIS STRUCTURE. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG3350 | 15-17 (%) | |
2 | 1 | reservoir | citrate | 0.1 (M) | |
3 | 1 | reservoir | 50 (mM) | or 50mM Li2SO4 |