1ZQJ
DNA POLYMERASE BETA (POL B) (E.C.2.7.7.7) COMPLEXED WITH SEVEN BASE PAIRS OF DNA; SOAKED IN THE PRESENCE OF CACL2 (15 MILLIMOLAR) AND MGCL2 (15 MILLIMOLAR)
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1996-03-14 |
Detector | SDMS |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 178.052, 57.629, 48.176 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.300 |
R-factor | 0.206 |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | 1zqg |
RMSD bond length | 0.016 |
RMSD bond angle | 2.800 |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | TNT (V. 5-D) |
Refinement software | TNT (5-D) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 5760 | |
Completeness [%] | 74.0 | 79 |
Redundancy | 1.9 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | THIS ENTRY DESCRIBES THE STRUCTURE THAT RESULTED WHEN A COCRYSTAL OF HUMAN DNA POLYMERASE BETA COMPLEXED WITH 7 BASE PAIRS OF DNA (SEE ENTRY 9ICJ AND REFERENCE 1) HAD BEEN SOAKED IN THE FOLLOWING SOLUTION FOR 12 HOURS: PEG 3350, 16% IMIDAZOLE, 100 MILLIMOLAR, PH 6.5 CACL2, 15 MILLIMOLAR MGCL2, 15 MILLIMOLAR SEE REFERENCE 3 FOR DETAILS CONCERNING EXPERIMENTAL PROCEDURES, RESULTS, AND DISCUSSION FOR THIS STRUCTURE. |