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1ZQG

DNA POLYMERASE BETA (POL B) (E.C.2.7.7.7) COMPLEXED WITH SEVEN BASE PAIRS OF DNA; SOAKED IN THE PRESENCE OF A SODIUM-FREE ARTIFICIAL MOTHER LIQUOR AT PH 6.5

Experimental procedure
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]298
Detector technologyAREA DETECTOR
Collection date1996-01-24
DetectorSDMS
Spacegroup nameP 21 21 2
Unit cell lengths179.044, 57.691, 48.334
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 3.100
R-factor0.162
Structure solution methodDIFFERENCE FOURIER
Starting model (for MR)8icb
RMSD bond length0.018
RMSD bond angle2.900
Data reduction softwareSDMS
Data scaling softwareSDMS
Phasing softwareTNT (V. 5-D)
Refinement softwareTNT (5-D)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0003.200
High resolution limit [Å]3.1003.100
Number of reflections8634
Completeness [%]89.085
Redundancy1.91.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
16.5THIS ENTRY DESCRIBES THE STRUCTURE THAT RESULTED WHEN A COCRYSTAL OF HUMAN DNA POLYMERASE BETA COMPLEXED WITH 7 BASE PAIRS OF DNA (SEE ENTRY 9ICJ AND REFERENCE 1) HAD BEEN SOAKED IN THE FOLLOWING SOLUTION FOR 7 DAYS: PEG 3350, 16% IMIDAZOLE, 100 MILLIMOLAR, PH 6.5 SEE REFERENCE 3 FOR DETAILS CONCERNING EXPERIMENTAL PROCEDURES, RESULTS, AND DISCUSSION FOR THIS STRUCTURE.

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