1ZQD
DNA POLYMERASE BETA (POL B) (E.C.2.7.7.7) COMPLEXED WITH SEVEN BASE PAIRS OF DNA; SOAKED IN THE PRESENCE OF CACL2 (150 MILLIMOLAR)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-03-05 |
| Detector | SDMS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 178.565, 57.826, 47.988 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.144 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 1zqg |
| RMSD bond length | 0.020 |
| RMSD bond angle | 3.100 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | TNT (V. 5-D) |
| Refinement software | TNT (5-D) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 4958 | |
| Completeness [%] | 73.0 | 71 |
| Redundancy | 1.8 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | THIS ENTRY DESCRIBES THE STRUCTURE THAT RESULTED WHEN A COCRYSTAL OF HUMAN DNA POLYMERASE BETA COMPLEXED WITH 7 BASE PAIRS OF DNA (SEE ENTRY 9ICJ AND REFERENCE 1) HAD BEEN SOAKED IN THE FOLLOWING SOLUTION FOR 24 HOURS: PEG 3350, 16% IMIDAZOLE, 100 MILLIMOLAR, PH 6.5 CACL2, 150 MILLIMOLAR SEE REFERENCE 3 FOR DETAILS CONCERNING EXPERIMENTAL PROCEDURES, RESULTS, AND DISCUSSION FOR THIS STRUCTURE. |
