1ZPW

Crystal structure of a hypothetical protein TT1823 from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-05
Detector	RIGAKU JUPITER 210
Wavelength(s)	1
Spacegroup name	I 2 2 2
Unit cell lengths	44.234, 55.964, 74.937
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.460 - 1.640
R-factor	0.18595
Rwork	0.185
R-free	0.21259
Structure solution method	MR, MAD
RMSD bond length	0.014
RMSD bond angle	1.587
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.700
High resolution limit [Å]	1.640	1.640
Number of reflections	11711
Completeness [%]	99.8	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	293	22% PEG 3350, 0.1M TrisCl pH 7.6, 0.2M NaF, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.6	293	22% PEG 3350, 0.1M TrisCl pH 7.6, 0.2M NaF, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.6	293	22% PEG 3350, 0.1M TrisCl pH 7.6, 0.2M NaF, VAPOR DIFFUSION, HANGING DROP, temperature 293K