1ZPU
Crystal Structure of Fet3p, a Multicopper Oxidase that Functions in Iron Import
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-20 |
Detector | CUSTOM-MADE |
Wavelength(s) | 1.37837 |
Spacegroup name | P 32 |
Unit cell lengths | 168.534, 168.534, 174.605 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.800 |
R-factor | 0.22721 |
Rwork | 0.226 |
R-free | 0.25742 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.411 |
Data reduction software | HKL-2000 |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 136782 | |
<I/σ(I)> | 14.9 | 2.3 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | sodium citrate, HEPES, ethylene glycol, ethanol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | sodium citrate, HEPES, ethylene glycol, ethanol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | sodium citrate, HEPES, ethylene glycol, ethanol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |