1ZP7
The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence specific cleavage.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-07-02 |
Detector | RIGAKU |
Wavelength(s) | 0.97 |
Spacegroup name | P 65 |
Unit cell lengths | 74.340, 74.340, 153.466 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.070 - 2.250 |
R-factor | 0.21925 |
Rwork | 0.217 |
R-free | 0.26943 |
Structure solution method | MIR |
RMSD bond length | 0.012 |
RMSD bond angle | 1.320 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.100 |
High resolution limit [Å] | 2.250 |
Number of reflections | 22690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | ||||
1 |