1ZOW
Crystal Structure of S. aureus FabH, beta-ketoacyl carrier protein synthase III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.900, 93.600, 110.000 |
| Unit cell angles | 90.00, 93.90, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hn9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.085 | 0.231 |
| Number of reflections | 84128 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 96.6 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 273 | PEG3K, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
