1ZON
CD11A I-DOMAIN WITHOUT BOUND CATION
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-02 |
| Detector | FUJI |
| Wavelength(s) | 0.9686, 0.9791, 0.9793, 0.9879 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.090, 63.690, 40.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.24300 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 * |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.110 |
| Number of reflections | 11039 |
| Completeness [%] | 96.4 |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | CD11a I domain | 10-20 (mg/ml) | |
| 2 | 1 | drop | EDTA | 2 (mM) | |
| 3 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
| 4 | 1 | reservoir | PEG3350 | 30-33 (%) | |
| 5 | 1 | reservoir | EDTA | 1 (mM) | |
| 6 | 1 | reservoir | Tris-HCl | 30 (mM) | |
| 7 | 1 | reservoir | beta-mercaptoethanol | 2 (mM) |
