1ZO2

Structure of nuclear transport factor 2 (Ntf2) from Cryptosporidium parvum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-05-11
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	43.516, 61.733, 85.543
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.160 - 1.600
R-factor	0.212
Rwork	0.212
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gyb
RMSD bond length	0.007
RMSD bond angle	1.200
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.300	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.046	0.269
Number of reflections	28649
<I/σ(I)>	35.7	5.2
Completeness [%]	91.8	56.2
Redundancy	6.7	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	PEG 3350, Ammonium phosphate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K