1ZNQ
Crystal Structure of Human Liver GAPDH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 124.500, 132.310, 86.037 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.570 - 2.500 |
Rwork | 0.211 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB code 1J0x |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 46609 | |
<I/σ(I)> | 14.17 | 1.63 |
Completeness [%] | 91.2 | 63.5 |
Redundancy | 5.5 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 18% w/v PEG 3350, 0.1M Sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |