1ZNO
Crystal Structure of VC702 from Vibrio Cholerae, Northeast Structural Genomics Consortium Target: VcP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-23 |
Detector | BRANDEIS - B4 |
Wavelength(s) | .97912 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 66.611, 88.118, 118.355 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.26290 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.131 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 23994 | |
Completeness [%] | 99.9 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 30% Peg 4000, .1M TRis pH 8.5, .2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 294K |