1ZN7

Human Adenine Phosphoribosyltransferase Complexed with PRPP, ADE and R5P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	47.019, 47.267, 47.522
Unit cell angles	76.59, 69.26, 61.89

Refinement procedure

Resolution	39.750 - 1.830
R-factor	0.148
Rwork	0.146
R-free	0.19000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.815
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.750	1.930
High resolution limit [Å]	1.830	1.830
Rmerge	0.069	0.387
Number of reflections	28504
<I/σ(I)>	9	1.9
Completeness [%]	96.0	93.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	15.0 % (V/V) glycerol, 25.5 % (w/V) polyethylene glycol 4000, 0.17 mol/L sodium acetate and 0.085 mol/L Tris-HCl pH 8.5, temperature 291K, VAPOR DIFFUSION, HANGING DROP, pH 8.50