1ZN2
Low Resolution Structure of Response Regulator StyR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8416 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.660, 36.546, 75.120 |
| Unit cell angles | 90.00, 100.32, 90.00 |
Refinement procedure
| Resolution | 25.300 - 2.910 |
| R-factor | 0.26871 |
| Rwork | 0.265 |
| R-free | 0.35232 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.410 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.300 | 2.950 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Number of reflections | 4795 | |
| Completeness [%] | 96.8 | 47.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 277 | PEG 4000, MgCl2, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
