1ZMO
Apo structure of haloalcohol dehalogenase HheA of Arthrobacter sp. AD2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-09 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93 |
Spacegroup name | P 1 |
Unit cell lengths | 64.980, 77.720, 111.422 |
Unit cell angles | 97.29, 89.97, 112.98 |
Refinement procedure
Resolution | 20.050 - 2.000 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pwx |
RMSD bond length | 0.020 |
RMSD bond angle | 2.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.090 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 120343 | |
Completeness [%] | 89.4 | 89.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 22% w/v PEG 8000, 100 mM MES buffer, 100 mM Magnesium Acetate, 50 mM Sodium Formate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |