1ZME
CRYSTAL STRUCTURE OF PUT3/DNA COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07-02 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 6 |
| Unit cell lengths | 121.650, 121.650, 39.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.29200 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.753 |
| Data reduction software | MOSFLM |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 11024 |
| Completeness [%] | 95.0 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.0 MM DIMER PROTEIN + 1.5 MM DNA DUPLEX PREPARED. 1.0 MILLILITER (0.1 M MES BUFFER, PH 6.5 + 10 MM MG CL2 + 20 MM NA CL + 20 % PEG 4K) RESERVOIR. HANGING DROP, LINBRO BOX, 20 DEGREE C. WITHIN 5 DAYS, 0.2 X 0.3 X 0.4 MM CRYSTALS., vapor diffusion - hanging drop, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES BUFFER | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 1 | PEG 4000 | ||
| 5 | 1 | 2 | MES BUFFER | ||
| 6 | 1 | 2 | MGCL2 | ||
| 7 | 1 | 2 | NACL | ||
| 8 | 1 | 2 | PEG 4000 |
