1ZMA
Crystal Structure of the Bacterocin Transport Accessory Protein from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-08 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.006, 69.658, 29.621 |
| Unit cell angles | 90.00, 112.27, 90.00 |
Refinement procedure
| Resolution | 25.500 - 1.250 |
| R-factor | 0.13138 |
| Rwork | 0.127 |
| R-free | 0.16686 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.434 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.500 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.071 | 0.225 |
| Number of reflections | 24991 | |
| <I/σ(I)> | 14.9 | 6.8 |
| Completeness [%] | 89.8 | 51.6 |
| Redundancy | 7 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | PEG 3350, HEPES, Sodium Chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
