1ZM5
Conjugative Relaxase TRWC in complex with ORIT dna, cooper-bound structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97855 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 91.077, 91.077, 205.551 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.680 - 2.500 |
| R-factor | 0.261 |
| Rwork | 0.233 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omh |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.520 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 24.680 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.104 |
| Number of reflections | 18252 |
| Redundancy | 21.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | PEG MME 2000, Sodium acetate, Ammonium sulfate, Cooper (II) chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG MME 2000 | ||
| 2 | 1 | 1 | Sodium acetate | ||
| 3 | 1 | 1 | Ammonium sulfate | ||
| 4 | 1 | 1 | Cooper (II) chloride | ||
| 5 | 1 | 2 | Sodium acetate | ||
| 6 | 1 | 2 | PEG MME 2000 |
