1ZM4
Structure of the eEF2-ETA-bTAD complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.952 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 326.950, 68.580, 190.200 |
| Unit cell angles | 90.00, 103.42, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.247 |
| Rwork | 0.246 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 1n0u and 1aer |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.430 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.150 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 91645 | |
| <I/σ(I)> | 11.8 | 4.8 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | PEG 6000, MPD, HEPES, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
