1ZLL
NMR Structure of Unphosphorylated Human Phospholamban Pentamer
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE | 500 | |
| 2 | Bruker | DMX | 600 | |
| 3 | Bruker | AVANCE | 750 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | TROSY-HNCA, HNCACB, HNCACO |
| 2 | 1 | 2 | measurement of 1H-15N, 13C'-13CA, and 13C'-15N RDCs using 3D HNCO based experiments |
| 3 | 1 | 3 | HCCONH, CCONH |
| 4 | 1 | 3 | 15N-edited NOESY, 13C-edited NOESY |
| 5 | 1 | 4 | 13C-HSQC for methyl stereospecific assignment |
| 6 | 1 | 5 | Double 13C-filtered, 15N-edited NOESY |
| 7 | 1 | 6 | 3D 15N-edited NOESY |
| 8 | 1 | 3 | 2D spin-echo difference experiments for measuring 3-bond J(NCg) and J(CCg) couplings |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6 | ambient | 303 |
Conformers
| Conformers Calculated Total Number | 30 |
| Conformers Submitted Total Number | 20 |
