1ZLJ
Crystal Structure of the Mycobacterium tuberculosis Hypoxic Response Regulator DosR C-terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-28 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.069, 60.488, 74.226 |
| Unit cell angles | 89.90, 89.91, 90.99 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.21100 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.318 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.108 | 0.412 |
| Number of reflections | 37716 | |
| <I/σ(I)> | 14.7 | 3.7 |
| Completeness [%] | 97.3 | 91.1 |
| Redundancy | 7.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | PEG 5000mme, ammonium sulfate, sodium chloride, MES, glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 5.50 |
