1ZLB
Crystal structure of catalytically-active phospholipase A2 in the absence of calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.80 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 39.964, 53.794, 89.816 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 0.970 |
| R-factor | 0.168 |
| Rwork | 0.151 |
| R-free | 0.19610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.040 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 0.990 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Rmerge | 0.068 | 0.477 |
| Number of reflections | 57413 | |
| Completeness [%] | 99.3 | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG 3350, NaCl, EDTA, tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
