1ZL9
Crystal Structure of a major nematode C.elegans specific GST (CE01613)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9160 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.960, 88.272, 93.596 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.010 |
| R-factor | 0.1522 |
| Rwork | 0.151 |
| R-free | 0.20912 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tw9 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.748 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.286 |
| Number of reflections | 33450 | |
| <I/σ(I)> | 28.3 | 5.4 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 65% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
